Use of Mass Spectrometry to Assist Production of SARS-CoV-2 Spike Protein Characterisation
Peak Proteins - read the recently published Sciex COVID Technote written in collaboration with Sciex.
Peptide mapping analysis of COVID-19 spike protein using the SCIEX X500B QTOF System and Protein Metrics Inc. software enables the confirmation of induced protein variants.
Authors:
Raquel Faba-Rodriguez and Rachel Rowlinson from Peak Proteins and
Sibylle Heidelberger, Chris Nortcliffe and Stephen Lock from SCIEX, UK
Abstract:
In this work, mass spectrometry has been used in assessing purity during protein production experiments. Both wild type (WT) and mutant forms of SARS-CoV-2 spike protein were expressed in a HEK293 system and were confirmed by analysis with the SCIEX X500B System. Rapid and efficient characterization was performed by using automated data analysis with Byos software (Protein Metrics Inc.).
